Pathogen-Imprinted Polymer Film Integrated probe/Ti3C2Tx MXenes Electrochemical Sensor for Highly Sensitive Determination of Listeria Monocytogenes

As one of the most hazardous and deadliest pathogens, Listeria monocytogenes (LM) posed various serious diseases to human being, thus designing effective strategy for its detection is great significance. In this work, by preparing Ti3C2Tx MXenes nanoribbon (Ti3C2TxR) as carrier selecting thionine (Th) acted simultaneously signal probe functional monomer, a LM pathogen-imprinted polymers (PIP) integrated electrochemical sensor was design monitor first time, that carried out through electropolymerization Th on Ti3C2TxR/GCE surface in existence LM. Upon eluting templates from imprinted cavities, fabricated PIP/Ti3C2TxR/GCE can rebound cells effectively. By recording peak current response signal, it be weakened when cell re-bound cavity PIP/Ti3C2TxR/GCE, absolute values change increase with increasement concentrations. After optimizing three key parameters, considerable low analytical limit (2 CFU mL−1) wide linearity (10–108 were achieved. addition, experiments demonstrated PIP/Ti3C2TxR offers satisfactory selectivity, reproducibility stability.